* Strong detergent lysis buffer
* pH 7.4, 1X solution
* Use to solubilize menbrane proteins and release cytosolic components
* Convenient pre-formulated solution.
Background :
RIPA buffer is commonly used to extract proteins for immunoprecipitation and/or Western blotting. RIPA has strong extraction properties that solubilizes and extracts proteins from membranes and releases cytoplasmic cellular components. RIPA buffer is mildly denaturing since it contains ionic detergent.