Storage condition : 4 ℃

Introductions
In vitro cultures may not represent true differential protein expression patterns, as a result, in vivo, formalin-fixed, paraffin-embedded tissues represent a comprehensive source of protein expression profiles especially in clinical studies or studies of diseases such as cancer. These samples enable the course of disease — e.g., before and after therapy — to be examined . FFPE Protein Extraction Kit provides optimized conditions for extracting total protein from FFPE or formalin-fixed tissue sections. After rapid 5-minute de-paraffinizing by de-paraffinization reagent, the samples are incubated in an optimized extraction buffer at two different temperatures in a process that reverses formalin crosslinking and untangles the protein molecules. After a centrifugation step, the supernatant containing the released proteins is precipitated, recovered and can be used for western blot , SDS-PAGE and other applications. The kit can perform 50 mini-preps.

Components
Pretreat Reagent 1 100 ml
Preteat Reagent 2 25 ml
Extraction Buffer 5 ml
Precipitation Reagent 75 ml
DTT 80 mg

Note : Before use, add DTT powder into Extraction buffer. Vortex until solution is completely dissolved. After usage, store Extraction Buffer at 4°C.