Introduction :

The tissue Protein extraction Kit provides an optimized cell lysis buffer, protease inhibitor cocktail, and Phosphatase inhibitor for convenient extraction of mammalian proteins from cultured cells and tissue samples, under non-denaturing conditions. Total protein isolated by this kit maintain biological activity and can be used for many downstream applications including SDS-PAGE, Western Blotting, IP, Pull Down, Gel Mobility Shift, protein assays and so on. The kit is sufficient to extract proteins from 50 x 10⁷ mammalian cells or 50 x 200 mg of tissues sample. 

Compositions :

Lysis Buffer 50 ml Protease Inhibitor 50 ul Phosphatase Inhibitor 250 ul PMSF 500 ul.

Storage and Transportation :

Transportation at room temperature. Upon receipt, store Lysis buffer at 4 ℃. Keep Protease Inhibitor Phosphatase Inhibitor, and PMSF at -20 ℃.