The PFU Master Mix is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mixes contain all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PFU Master Mix is supplied as a 5 X concentrated ready-to-use mix, that is a mixture of recombinant 5 u of PFU DNA polymerase, reaction buffer, MgCl2 (final concentration 2 mM), 250 μM dNTP and enzyme stabilizer sufficient to allow efficient amplification of template in PCR.