pGen2.1 vector is a Human cytomegalovirus (CMV) promoter-based mammalian gene expression vector. CMV promoter is one of the strongest promoters described. Based on RNA polymerase II system, CMV promoter drives higher-level constitutive expression of genes in a variety of mammalian cell lines. A target gene can be conveniently cloned into pGEN2.1 vector at multiple cloning sites (MCS). The target protein is expressed with the FLAG tag at the N-terminal. This tag will facilitate detection and purification of the target protein using commercially available tag-specific antibodies. This vector also contains a selectable marker for mammalian cells, neomycin phosphotransferase gene, under SV40 promoter. pGen2.1 vector can be used for either transient expression or stable expression in the presence of the antibiotic G418.
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