GenScript THETM HA Tag Antibody [HRP], mAb, Mouse is THETM HA Tag Antibody, mAb, Mouse (A01244) conjugated with Horseradish peroxidase (HRP). The conjugated antibody is suitable for detecting the expression level of HA tagged fusion proteins.
Ig 亞類
IgG1, k
儲存條件
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
特異性
THETM HA Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
濃度
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
免疫抗原
A synthetic peptide (YPYDVPDYA) coupled to KLH
純化方法
Protein A affinity column
複溶方法
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
應用
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.1-0.5 µg/ml Western blot: 0.1-0.5 µg/ml Other applications: user-optimized
融合標籤
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.