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基本描述
GenScript THETM HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our THETM elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
Ig 亞類
IgG1
儲存條件
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
特異性
THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
應用
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Immunoprecipitation: 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry: 1 µg/ml Other applications: user-optimized
融合標籤
Spleen cells fused with SP2/0-Ag14 mouse myeloma cells.
宿主
Mouse
注釋
GenScript can offer this product per customer's reuqest including product size, buffer components,etc.