Full Name

THE^TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

Abbreviated name-1

anti Flag-tag mAb; anti DYKDDDDK-tag mAb

基本描述

GenScript THE^TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THE^TM DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.

Ig 亞類

IgG2b, k

儲存條件

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

特異性

THE^TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins.

濃度

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

免疫抗原

A synthetic peptide (DYKDDDDK) coupled to KLH

純化方法

Protein A affinity column

複溶方法

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

應用

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

融合標籤

Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells

共軛物

Biotin

宿主

Mouse

技術操作手册

1