Full Name

THE^TM c-Myc Antibody [HRP], mAb, Mouse

Abbreviated name-1

Mouse Anti-c-Myc [HRP] mAb;

基本描述

GenScript THE^TM c-Myc Antibody [HRP], mAb, Mouse is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.

Ig 亞類

Mouse IgG2a

儲存條件

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

特異性

GenScript THE^TM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.

濃度

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

免疫抗原

A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH

純化方法

Protein A affinity column

複溶方法

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

應用

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized

融合標籤

Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.

共軛物

Horseradish peroxidase (HRP)

宿主

Mouse

技術操作手册

1