HJ Koo.,et al. Suites of Terpene Synthases Explain Differential Terpenoid Production in Ginger and Turmeric Tissues.PLoS One.2012Dec;7(12):e51481
Campos SK.,et al. Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration.J Virol.2012Apr;86(8):4169-81
Zhu M.,et al. Investigation of the influence of Arg555Trp and Thr538Pro TGFBI mutations on C-terminal cleavage and cell endoplasmic reticulum stress.Mol Vis.2012Jan;18:1156-64
Natalya L. Teterina.,et al. Analysis of Poliovirus Protein 3A Interactions with Viral and Cellular Proteins in Infected Cells.J Virol.2011May;85(9):4284 - 4296
Steven M. Markus.,et al. Quantitative analysis of Pac1/LIS1â€mediated dynein targeting: Implications for regulation of dynein activity in budding yeast.Cytoskeleton (Hoboken).2011Mar;68(3):157-74
Floria Faradji.,et al. Drosophila melanogaster Cyclin G coordinates cell growth and cell proliferation.Cell Cycle.2011Mar;10(5):805-18
Daniel K. Stringer and Robert C. Piper..,et al. A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination.J Cell Biol.2011Jan;192(2):229 - 242
基本描述
The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
主要特性
· GenScript THETM c-Myc Tag Antibody, mAb, Mouse has high sensitivity, better than the antibody on the market.
· GenScript THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) can recognize N-terminal, C-terminal and internal c-Myc-tag in fusion proteins.
Ig 亞類
IgG2a, k
儲存條件
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
特異性
THETM c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.
Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
應用
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05 µg/ml-0.2 µg/ml Western blot: 0.05 µg/ml-0.5 µg/ml Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 0.9 µg of this antibody is mixed with diluted WB solution (dilute 1.5 ml WB with 1.5 ml of PBST). Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Other applications: user-optimized
融合標籤
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells