產品名稱 : Trypsin-EDTA Solution 1x (IN D-PBS) 產品編號 : TCL014 產品規格 : 100 mL x5 廠 牌 : Biokit 0.02% EDTA in Dulbecco’s phosphate buffered saline.
詳細資料
Trypsin is a serine protease derived from porcine pancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells. Ethylenediaminetetraacetic acid (EDTA), a chelating agent is often added to enhance enzymatic activity of trypsin solution. EDTA acts by neutralizing calcium and magnesium ions that enhance cell to cell adhesion.
BCL007 is 0.25% Trypsin and 0.02% EDTA in Dulbecco’s phosphate buffered saline.