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IMAC-μSphere
產品名稱 IMAC-μSphere
產品編號 P002
產品規格 5mL
廠    牌 Biokit

詳細資料

IMAC-μSphere                                   

This is an affinity purification matrix for His-Tag Fusion proteins and other metal proteins.
polymer μSphere-based chromatographic matrix High Flow Rate,High capacity and high performance 

IMAC μSphere 6 cess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins

l Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity

l Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion

l As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.

l Available in the convenient and time-saving prepacked HiTrap and HiPrep formats.
 

IMAC μSphere 6 is a member of the range of IMAC chromatography media for capture and intermediate purification.

IMAC μSphere 6 is composed of highly cross-linked beads modified with a novel chelating ligand immobilized to the base matrix. IMAC μSphere 6 is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.

IMAC μSphere 6 is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. 
As member of the BioProcess media range, IMAC μSphere 6 meets industrial demands with security of supply and comprehensive technical and regulatory support

 

Appendix:

Summary and Comparison of Matrices  Extensively and Successfully Used in Protein Purification and Analysis

Category

 

Particle size

(μm

pH Stability

Max Pressure

Max Flow Rate(cm/h)

Source 15

PS/DVB

15

1~14

10MPa

900

Source 30

PS/DVB

30

1~14

10

2000

Resource

PS/DVB

15

1~14

10

1800

MonoBeads

rous PS/DVB

10

1~14

10

1200

MiniBeads

Monodisperse PS/DVB

3, non porous

1~14

10

 

Fast Flow

6% cross-linked agarose

45-165, avr. 90

2~13

0.3

300

Capto

Highly cross-linked agarose with dextran surface extender

90 (d50V)

2~13

0.3

1200

HP

6% cross-linked agarose

24-44, avr. 34

1~14

0.3

150

mSphere

Polyacrylate or PS/DVB

3, 10, 15, 30, 50, 100

1~14

10

1200~2000

售價:8300
   數量:


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