IMAC-μSphere
This is an affinity purification matrix for His-Tag Fusion proteins and other metal proteins.
polymer μSphere-based chromatographic matrix High Flow Rate,High capacity and high performance
IMAC μSphere 6 cess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins
l Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
l Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
l As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
l Available in the convenient and time-saving prepacked HiTrap and HiPrep formats.
IMAC μSphere 6 is a member of the range of IMAC chromatography media for capture and intermediate purification.
IMAC μSphere 6 is composed of highly cross-linked beads modified with a novel chelating ligand immobilized to the base matrix. IMAC μSphere 6 is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC μSphere 6 is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC μSphere 6 meets industrial demands with security of supply and comprehensive technical and regulatory support
Appendix:
Summary and Comparison of Matrices Extensively and Successfully Used in Protein Purification and Analysis
Category
|
|
Particle size
(μm)
|
pH Stability
|
Max Pressure
|
Max Flow Rate(cm/h)
|
Source 15
|
PS/DVB
|
15
|
1~14
|
10MPa
|
900
|
Source 30
|
PS/DVB
|
30
|
1~14
|
10
|
2000
|
Resource
|
PS/DVB
|
15
|
1~14
|
10
|
1800
|
MonoBeads
|
rous PS/DVB
|
10
|
1~14
|
10
|
1200
|
MiniBeads
|
Monodisperse PS/DVB
|
3, non porous
|
1~14
|
10
|
|
Fast Flow
|
6% cross-linked agarose
|
45-165, avr. 90
|
2~13
|
0.3
|
300
|
Capto
|
Highly cross-linked agarose with dextran surface extender
|
90 (d50V)
|
2~13
|
0.3
|
1200
|
HP
|
6% cross-linked agarose
|
24-44, avr. 34
|
1~14
|
0.3
|
150
|
mSphere
|
Polyacrylate or PS/DVB
|
3, 10, 15, 30, 50, 100
|
1~14
|
10
|
1200~2000
|
|