BstX2 I (Xho II)-500U 產品名稱 : BstX2 I (Xho II) 產品編號 : E229 產品規格 : 500U 廠    牌 : Biokit Recognition site R↑GATCY YCTAG↓R Source Isolated from E.coli strain that carries the cloned BstX2I gene from Bacillus stearothermophilus X2 Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y 75 - 100 100 0 - 10 10 - 25 25 - 50 Optimal temperature 60oC Storage conditions 10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20oC. Ligations After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60oC. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity Not blocked by Dam-methylation (GmATC): RGATCY Inactivation 20 minutes under 80oC

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