Xba I-1000U 產品名稱 : Xba I 產品編號 : E141 產品規格 : 1000U 廠    牌 : Biokit 詳細資料   Recognition site T↑CTAGA AGATC↓T Source E.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii Assayed on Lambda DNA (dam-/HindIII-digest) Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y 75 - 100 75 - 100 100 50 - 75 75 - 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20oC. Ligations After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37oC. Reagents Supplied with Enzyme 10 X SE-buffer o, BSA Methylation sensitivity Blocked by overlapping Dam-methylation (GmATC): TCTAGATC Inactivation 20 minutes under 65oC

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