Sph I-300U 產品名稱 ： Sph I 產品編號 ： E129 產品規格 ： 300 U 廠    牌 ： Biokit 詳細資料 Recognition site GCATG↑C C↓GTACG Source E.coli strain that carries the cloned Sph I gene from Streptomyces phaeochromogenes Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y 25 - 50 100 75 - 100 75 - 100 50 - 75 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol Store at -20oC. Ligations After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37oC Reagents Supplied with Enzyme 10 X SE-buffer G, BSA Methylation sensitivity not tested Inactivation 20 minutes under 65oC

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