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PspX I-1000U |
產品名稱 : PspX I
產品編號 : E478
產品規格 : 1000 U
廠 牌 : Biokit |
Recognition site |
VC↑TCGAGB
BGAGCT↓CV
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Source |
E.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1 |
Assayed on |
Lambda DNA (HindIII-digest) |
Unit |
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer |
Y (33 mM Tris-acetate (pH 7.9 at 25oC); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA |
Enzyme activity (%) |
B |
G |
O |
W |
Y |
50 - 75 |
50 - 75 |
25 - 50 |
75 - 100 |
100 |
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Optimal temperature |
37oC
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Storage conditions |
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2 -mercaptoethanol, 200 μg/ml BSA; 50% glycerol. Store at -20oC. |
Ligations |
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis |
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37oC. |
Reagents Supplied with Enzyme |
10 X SE-buffer Y, BSA |
Methylation sensitivity |
not tested |
Inactivation 20 minutes under |
80oC
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購物說明: |
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