PspC I (Pmac I)-10000U 產品名稱 ： PspC I (Pmac I) 產品編號 ： E476 產品規格 ： 10000 U 廠    牌 ： Biokit Recognition site CAC↑GTG GTG↓CAC Source Pseudomonas species C Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y 100 50 - 75 0 - 10 0 - 10 50 - 75 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Storage temperature: -20ºC. For long term storage (more than 30 days), store at -70ºC. Ligations After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Ligation is better in presence of 10% PEG. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37oC. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA Methylation sensitivity not tested Inactivation 20 minutes under 65oC

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