Kpn I-2000U 產品名稱 ： Kpn I 產品編號 ： E079 產品規格 ： 2000 U 廠    牌 ： Biokit 詳細資料   Recognition site GGTAC↑C C↓CATGG Source Isolated from an E.coli strain that carries the cloned Kpn I gene from Klebsiella pneumonia Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer B (10 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y 100 25 - 50 25 - 50 25 - 50 75 - 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20oC. Ligations After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37oC. Reagents Supplied with Enzyme 10 X SE-buffer B, BSA Methylation sensitivity Not blocked by overlapping Dcm methylation (CmCWGG): GGTACCWGG Inactivation 20 minutes under 80oC

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