EcoR I-5000U 產品名稱 ： EcoR I 產品編號 ： E058 產品規格 ： 5000 U 廠    牌 ： Biokit 詳細資料 Recognition site G↑AATTC CTTAA↓G Source E.coli strain that carries the cloned EcoR I gene from Escherichia coli Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer SE-buffer EcoRI (100 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y 50 - 75 75 - 100 75 - 100 75 - 100 50 - 75 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerolerol; Store at -20oC. Ligations After 40-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37oC. Reagents Supplied with Enzyme 10 X SE-buffer EcoRI, BSA Methylation sensitivity not tested Inactivation 20 minutes under 65oC

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