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EcoR I-1000U |
產品名稱 : EcoR I
產品編號 : E057
產品規格 : 1000 U
廠 牌 : Biokit |
詳細資料 |
Recognition site |
G↑AATTC
CTTAA↓G
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Source |
E.coli strain that carries the cloned EcoR I gene from Escherichia coli |
Assayed on |
Lambda DNA |
Unit |
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer |
SE-buffer EcoRI (100 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA |
Enzyme activity (%) |
B |
G |
O |
W |
Y |
50 - 75 |
75 - 100 |
75 - 100 |
75 - 100 |
50 - 75 |
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Optimal temperature |
37oC
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Storage conditions |
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerolerol; Store at -20oC. |
Ligations |
After 40-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis |
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37oC. |
Reagents Supplied with Enzyme |
10 X SE-buffer EcoRI, BSA |
Methylation sensitivity |
not tested |
Inactivation 20 minutes under |
65oC
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購物說明: |
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