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BstC8 I (Cac8 I)-200U |
產品名稱 : BstC8 I (Cac8 I)
產品編號 : E305
產品規格 : 200 U
廠 牌 : Biokit |
Recognition site |
GCN↑NGC
CGN↓NCG
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Source |
Bacillus stearothermophilus C8 |
Assayed on |
Lambda DNA |
Unit |
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl. |
Optimal SE-buffer |
Y (33 mM Tris-acetate (pH 7.9 at 25oC); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) |
B |
G |
O |
W |
Y |
10 - 25 |
25 - 50 |
50 - 75 |
75 - 100 |
100 |
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Optimal temperature |
55oC
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Storage conditions |
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20oC. |
Ligations |
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis |
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 55oC. |
Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Methylation sensitivity |
not tested |
Inactivation 20 minutes under |
80oC
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購物說明: |
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