Bgl II-1000U 產品名稱 ： Bgl II 產品編號 ： E027 產品規格 ： 1000 U 廠    牌 ： Biokit 詳細資料 Recognition site A↑GATCT TCTAG↓A Source Isolated from an E.coli strain that carries the cloned Bgl II gene from Bacillus globigii Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y 0 - 10 10 - 25 100 25 - 50 10 - 25 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20oC. Ligations After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37oC. Reagents Supplied with Enzyme 10 X SE-buffer O Methylation sensitivity Not blocked by overlapping dam methylation (GmATC) AGATCT Inactivation 20 minutes under 80oC

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