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Bme18 I (Ava lI)-1000U |
產品名稱 : Bme18 I (Ava lI)
產品編號 : E029
產品規格 : 1000 U
廠 牌 : Biokit |
Recognition site |
G↑GWCC
CCWG↓G
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Source |
Bacillus megaterium 18 |
Assayed on |
Lambda DNA |
Unit |
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer |
O (50 mM Tris-HCl (pH 7.6 at 25oC); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
Enzyme activity (%) |
B |
G |
O |
W |
Y |
10 - 25 |
25 - 50 |
100 |
75 - 100 |
10 - 25 |
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Optimal temperature |
37oC
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Storage conditions |
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20oC. |
Ligations |
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis |
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37oC. |
Reagents Supplied with Enzyme |
10 X SE-buffer O |
Methylation sensitivity |
Cleaved of DNA is impaired by overlapping dcm-methylation (CmCWGG): GGWCCWGG. |
Inactivation 20 minutes under |
65oC
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購物說明: |
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