Aat II-500u 產品名稱 ： Aat II  產品編號 ： E287 產品規格 ： 500 U 廠    牌 ： Biokit 詳細資料   Recognition site GACGT↑C C↓TGCAG Source E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti Assayed on Lambda DNA Unit One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme activity (%) B G O W Y 10 - 25 25 - 50 10 - 25 25 - 50 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -200C. Ligations After 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C); . Reagents Supplied with Enzyme 10 X SE-buffer Y Methylation sensitivity not tested Inactivation 20 minutes under 65oC

購物說明：