Isolated from E.coli strain that carries the cloned DNA ligase gene from bacteriophage T4
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'phosphate and 3'hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.
Applications:
- cloning of restriction fragments;
- joining linkers and adapters to blunt-ended DNA
Unit
One unit is defined as the amoumt of enzyme required to give 50% ligation of Hind III fragments of Lambda DNA (5'DNA termini concentration of 0.12uM[300ug/ml] in 20ul of 1 x T4 DNA Ligase Reaction Buffer in 30 minutes at 16oC. ATP is an essential cofactor for the reaction
Reaction buffer
SE-buffer DNA ligase T4, (50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.)
Optimal temperature
16oC
Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20oC
Quality control
Free of contaminating exonuclease and endonuclease
相關產品列表
購物說明：