Storage condition : 4 ℃
Introductions :
The Plant Protein Extraction Kit provides rapid recovery (as few as 20 minutes) of soluble
proteins from plant tissue samples. The final protein extract is compatible with many downstream applications, including SDS-PAGE, 2-D gel electrophoresis, Western blotting, activity assays andaffinity purification. The Kit includes three components: an organic solution to lyse plant cells, an aqueous buffer to gently solubilize proteins and a protein-stabilizing reagent. The Kit has been tested on leaves, roots, flowers and seeds from various species, including Arabidopsis, tobacco, spinach, peas and soybeans. Extremely fibrous tissue samples, such as woody stems, may require additional mechanical grinding by devices not included in the kit. Depending on the particular application, additional components, such as phosphatase inhibitor and chelating agents may be added to the buffer. The kit is sufficient to extract protein from either 20 × 40 mg of fresh/frozen plant tissue or 20 × 10 mg of dried plant tissue.
Components :
Solution A 20 ml
Solution B 20 ml
DTT(1M) 0.1 ml
Protease Inhibitor 25 ul
Storage :
Transportation at room temperature. Upon receipt, store DTT(1M) and Protease inhibitor at -20℃, Solution A and B at room temperature. Solution B must be kept away from light. The kit has one year expiration.
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