DH5a is the most popular E.coli strain for every day cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition,DH5a carries recA1 mutation that eliminates homologus recombination ensuring insert stability. It is also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the trnasformation of large plasmids and two-hybrid system.