Recommended use : 5µl HealthView Nucleic Acid Stain for 100 mL agarose gel solution

Storage : Store at  4 ^oC

Description :

HealthView Nucleic Acid Stain is a new and safe nucleic acid stain reagent. It can be used to replace the ethidium bromide stain (EtBr) for detecting double- strand DNA, single-strand DNA and RNA in agarose gels. When HealthViewTM Nucleic Acid  Stain  bound  to  dsDNA,  it  emits   green  fluorescence  or  red fluorescence when bound to ssDNA or RNA. HealthViewTM Nucleic Acid Stain is as  sensitive  as  EtBr,  and  the  stain in protocol  is  also  similar  to  EtBr. HealthViewTM Nucleic Acid Stain is unlike the EtBr, know as a strong mutagen, it causes fewer mutations in Ames test, and has negative results in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test, too. So HealthViewTM Nucleic Acid Stain is a better choice instead of EtBr for detecting nucleic acid in agarose gels.

Notes :

1.  The thickness of gel should be less than 0.5 cm since thick gels may decrease sensitivity.
2.  Repeated melt of gels containing  HealthViewTM Nucleic Acid Stain may cause low sensitivity.
3.  HealthViewTM Nucleic Acid Stain allows visualization of DNA (> 50 ng) in agarose gels under visible light. This eliminates the need for exposure to UV light, which can nick and damage  DNA. The intact DNA fragments purified  from  agarose  gel  can  increase  the  efficiency  of  subsequent molecular  biology  manipulations  such  as  cloning,  transformation  and transcription.