Specifications :  

* Strong detergent lysis buffer
* pH 7.4, 1X solution
* Use to solubilize menbrane proteins and release cytosolic components
* Convenient pre-formulated solution.

Background : 

RIPA buffer is commonly used to extract proteins for immunoprecipitation and/or Western blotting. RIPA has strong extraction properties that solubilizes and extracts proteins from membranes and releases cytoplasmic cellular components. RIPA buffer is mildly denaturing since it contains ionic detergent. 

Contents :
 
1X RIPA
1. 50mM Tris pH 7.4
2. 150mM NaCl
3. 0.1% SDS
4. 1% NP-40
5. 0.5% sodium deoxycholate